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Int.J.Curr.Microbiol.App.Sci (2013) 2(2):15-19

ISSN: 2319-7692 Volume 2 Number 2 (2013) pp.15 19 http://www.ijcmas.com

Original Research Article

Phytochemical and in vitro anti-diabetic activity of methanolic extract of Psidium guajava leaves R. Manikandan*, A.Vijaya Anand1 and G. Durai Muthumani2
Department of Chemistry, SSK Polytechnic College, Poolangulathupatti, Trichirappalli, Tamil Nadu, India. 1 P.G. Department of Biochemistry, M.I.E.T. Arts and Science College, Trichirappalli, Tamil Nadu, India 2 Department of Biochemistry, Kanchi Shri Krishna College of Arts and Science, Kilambi, Kancheepuram -631 551 Tamil Nadu, India. *Corresponding author: [email protected] ABSTRACT The intestinal digestive enzymes alpha-glucosidase and alpha-amylase are plays a vital role in the carbohydrate digestion. One antidiabetic therapeutic approach reduces the post prandial glucose level in blood by the inhibition of alpha-glucosidase and alpha-amylase enzymes. These can be an important strategy in management of blood glucose. The aim of the present study was to investigate the phytochemical bioactive compounds of the methanolic extract of Psidium guajava leaves, its in vitro anti-diabetic activity. The assay results suggests that the presence of bioactive compounds, could be responsible for the versatile medicinal properties of this plant including diabetes, the extract exhibit the dose-dependent increase in inhibitory effect on alpha-glucosidase enzyme (upto 89.4%), and alpha-amylase enzyme (upto 96.3%). The current study proves that the antidiabetic activity of methanolic extract of Psidium guajava leaves by in vitro studies. *

Keywords

Psidium guajava; Phytochemical; alphaglucosidase; alpha-amylase

Introduction
Diabetes mellitus is a complex and a diverse group of disorders that disturbs the metabolism of carbohydrate, fat and protein. The number of diabetes mellitus cases has been increasing worldwide in recent years. In 2000, the world health organization estimated a total of 171 million of people with diabetes mellitus from the global population, and this report projected to increase to 366 million by 2030 (Wild et al., 2004). With a long course and serious complications often resulting in high deathrate, the treatment of diabetes spent vast amount of resources including medicines, diets, physical training and so on in all countries. Thus searching for a new class of compounds is essential to overcome diabetic problems. There is continuous search for alternative drugs (Syamsudin, 2010). 15

Int.J.Curr.Microbiol.App.Sci (2013) 2(2):15-19

Psidium guajava Linn. (Myrtaceae) is commonly known as Peru or Guava in English. The Psidium guajava leaves are used in the treatment of diarrhoea, cough, stomachache, dysentery and decoction of the leaves for cholera patients, toothache and gum boils. The leaves showed hypoglycemic, cardioprotective, myocardial depressant, antimicrobial, antispasmodic actions (Ross, 1999). Inhibition of alphaamylase and alpha-glucosidase enzymes can be an important strategy in management of post prandial blood glucose level in type 2 diabetes patient (Ali et al., 2006). Thus, objective of the present study is to investigate the phyto chemical, invitro antidiabetic activity of methanolic extract of Psidium guajava leaves.

reducing sugar, tannins, flavonoids, steroids and alkaloids by using standard phytochemical procedures (Evans, W.C and Evans, T. 2003).

In vitro methods employed in antidiabetic studies Inhibition of alpha-amylase enzyme A starch solution (0.1% w/v) was obtained by stirring 0.1g of potato starch in 100 ml of 16 mM of sodium acetate buffer. The enzyme solution was prepared by mixing 27.5 mg of alpha-amylase in 100 ml of distilled water. The colorimetric reagent is prepared by mixing sodium potassium tartarate solution and 3, 5 di nitro salicylic acid solution 96 mM. Both control and plant extracts were added with starch solution and left to react with alpha-...